重组人SOST蛋白-ACROBiosystems百普赛斯

Human SOST / Sclerostin Protein, His Tag

分子别名（Synonym）

SOST,VBCH

表达区间及表达系统（Source）

Human SOST, His Tag (HST-H5245) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Tyr 213 (Accession # AAI01087.1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Sclerostin（SOST）也被称为Sclerosteosis，VBCH，是一种分泌性糖蛋白，具有用于分泌的信号肽和C末端半胱氨酸结样（CTCK）结构域，属于Cerberus/DAN家族的骨形态发生蛋白（BMP）拮抗剂。硬骨素由骨细胞产生，对骨形成具有抗合成代谢作用。最近，硬骨素已被鉴定为与LRP5/6受体结合并抑制Wnt信号通路。在这些情况下，Wnt通路的抑制对骨形成具有拮抗作用（即硬骨素拮抗骨形成）。已经表明，SOST以高亲和力结合BMP-5、-6和-7，以低亲和力结合BMP-2和-4。甲状旁腺激素、机械负荷和包括抑瘤素M、心肌营养素-1和白血病抑制因子在内的细胞因子抑制了骨细胞产生硬化素。降钙素可以增加硬化素的产生。因此，成骨细胞的活性是由负反馈系统自我调节的。硬骨素突变与硬骨症综合征有关，硬骨素表达减少导致一种较轻的疾病，称为van Buchem病。