重组人IL-33蛋白-ACROBiosystems百普赛斯

Human IL-33 Protein, His Tag, premium grade

分子别名（Synonym）

IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2

表达区间及表达系统（Source）

Human IL-33 Protein, His Tag, premium grade (IL3-H52H7) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1).

Predicted N-terminus: His

重组人IL-33蛋白，His Tag，premium级（IL3-H52H7），专为临床前阶段设计，具有与GMP级重组人IL-33蛋白相同的活性和性能，可实现从临床前开发到临床阶段的无缝过渡。在原材料安全不是首要任务的早期开发阶段，premium产品为GMP级产品提供了一种经济高效的替代品。通过在早期开发阶段使用Premium Grade产品，您可以轻松过渡到临床和商业阶段，而无需重新验证原材料和修改制造工艺。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素33（IL-33）被称为C9orf26、DKFZp586H0523、DVS27、NF-HEV、NFEHEV、RP11-575C20.2，是一种属于IL-1超家族的细胞因子。IL-33诱导辅助性T细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞产生2型细胞因子。IL-33通过与受体ST2（又名IL1RL1）和IL-1受体辅助蛋白（IL1RAP）相互作用，激活NF-κB和MAP激酶信号通路中的细胞内分子，驱动极化Th2细胞产生2型细胞因子（如IL-5和IL-13），从而介导其生物学效应。在体内，IL-33诱导IL-4、IL-5和IL-13的表达，并导致粘膜器官的严重病理变化。