重组人CD47蛋白-ACROBiosystems百普赛斯

Human CD47 Protein, His Tag (MALS verified)

分子别名（Synonym）

CD47,MER6,IAP,OA3

表达区间及表达系统（Source）

Human CD47, His Tag (CD7-H5227) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3).

Predicted N-terminus: Gln 19

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.6 kDa. The protein migrates as 33-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）

白细胞表面抗原CD47也被称为抗原表面决定蛋白OA3、整合素相关蛋白（IAP）和蛋白MER6。CD47含有1个Ig样V型（免疫球蛋白样）结构域。CD47在正常成人组织中分布非常广泛。CD47通过充当血小板上THBS1的粘附受体在细胞粘附中发挥作用，并在整合素的调节中发挥作用。通过相似性，CD47在海马的记忆形成和突触可塑性中起着重要作用。CD47是SIRPA的受体，与SIRPA结合可阻止未成熟树突状细胞的成熟，并抑制成熟树突状细胞产生细胞因子。CD47与SIRPG的相互作用介导细胞间粘附，增强超抗原依赖性T细胞介导的增殖，并共刺激T细胞活化。