重组人HGF蛋白-ACROBiosystems百普赛斯

Human HGF Protein, His Tag, premium grade

分子别名（Synonym）

HGF,HPTA,SF

表达区间及表达系统（Source）

Human HGF, His Tag, premium grade (HGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).

Predicted N-terminus: Gln 32

该产品是在我们严格的质量控制体系下生产的，该体系包含一套全面的测试，包括无菌和内毒素测试。在早期临床前阶段，产品性能经过仔细验证和测试，以确保其与细胞培养用途或任何其他应用的兼容性。当准备过渡到后期临床阶段时，我们还提供定制的GMP蛋白质服务，以满足您的需求。我们将与您合作，根据您的要求定制和开发GMP级产品，该产品也符合细胞疗法细胞制造中使用的原材料和辅助材料的要求。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

肝细胞生长因子（HGF）是一种旁分泌细胞生长、运动和形态发生因子。通过与受体酪氨酸激酶MET结合并促进其二聚化来激活配体。肝细胞生长因子由间充质细胞分泌，在主要来源于上皮的细胞上起多功能细胞因子的作用。其刺激有丝分裂、细胞运动和基质侵袭的能力使其在血管生成、肿瘤发生和组织再生中起着核心作用。此外，HGF与多种癌症有关，包括肺癌、胰腺癌、甲状腺癌、结肠癌和乳腺癌。