重组人TIGIT蛋白-ACROBiosystems百普赛斯

Human TIGIT Protein, His Tag, active dimer (MALS verified)

分子别名（Synonym）

TIGIT,VSIG9,VSTM3

表达区间及表达系统（Source）

Human TIGIT, His Tag (TIT-H52H5) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).

Predicted N-terminus: Met 22

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 28.8 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

具有Ig和ITIM结构域的T细胞免疫受体（TIGIT）也称为V-set和含免疫球蛋白结构域的蛋白9（VSIG9）、V-set和跨膜结构域的蛋白质3（VSTM3），属于单程I型膜蛋白，含有免疫球蛋白可变结构域、跨膜结构区和基于免疫受体酪氨酸的抑制基序（ITIM）。TIGIT在外周记忆和调节性CD4+T细胞和NK细胞上以低水平表达，并在这些细胞激活后上调（在蛋白质水平）。TIGIT以高亲和力结合脊髓灰质炎病毒受体（PVR），导致IL10分泌增加和IL12B分泌减少，并通过促进成熟免疫调节树突状细胞的产生来抑制T细胞活化。