Human Activin RIIB / ACVR2B Protein, His Tag (MALS & SPR verified)
分子别名（Synonym）
ACVR2B,ACTRIIB,MGC116908
表达区间及表达系统（Source）
Human Activin RIIB, His Tag (ACB-H5226) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Thr 137 (Accession # Q13705-1).
Predicted N-terminus: Ser 19
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.5 kDa. The protein migrates as 20-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
激活素受体2B型（ACVR2B）也称为ActR IIB和MGC116908，ACVR2B是激活素2型受体。激活素是二聚体生长和分化因子，属于结构相关信号蛋白的转化生长因子-β（TGF-β）超家族。激活素通过受体丝氨酸激酶的异质复合物发出信号，所述受体丝氨酸激酶包括至少两种I型（I和IB）和两种II型（II和IIB）受体。这些受体都是跨膜蛋白，由具有半胱氨酸富集区的配体结合胞外结构域、跨膜结构域和具有预测丝氨酸/苏氨酸特异性的细胞质结构域组成。I型受体对信号传导至关重要；并且II型受体是结合配体和表达I型受体所必需的。I型和II型受体在配体结合后形成稳定的复合物，导致I型受体被II型受体磷酸化。II型受体被认为是组成型活性激酶。该基因编码激活素A IIB型受体，其对配体的亲和力是激活素II型受体的3-4倍。ACVR2B缺陷是常染色体4型内脏异轴畸形（HTX4）的病因。
