重组人ROR1蛋白-ACROBiosystems百普赛斯

Human / Cynomolgus / Rhesus macaque ROR1 Protein, His Tag (MALS verified)

分子别名（Synonym）

ROR1,NTRKR1

表达区间及表达系统（Source）

Human / Cynomolgus / Rhesus macaque ROR1, His Tag (RO1-H522y) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # Q01973-1). In the region Gln 30 - Glu 403, the AA sequence of Human, Cynomolgus and Rhesus macaque ROR1 are homologus.

Predicted N-terminus: Gln 30

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 43.9 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 30 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）

酪氨酸蛋白激酶跨膜受体ROR1也称为神经营养性酪氨酸激酶受体相关1（NTRKR1），属于蛋白激酶超家族或tyr蛋白激酶家族或ROR亚家族。ROR1包含1个FZ（卷曲）结构域，1个Ig样C2型（免疫球蛋白样）结构域，1个kringle结构域，1个蛋白激酶结构域。ROR1在早期胚胎发育过程中高水平表达。表达水平在第16天左右强烈下降，成人组织中只有非常低的水平。ROR1可以与酪蛋白激酶1ε（CK1ε）相互作用，激活磷酸肌醇3激酶介导的AKT磷酸化和cAMP反应元件结合蛋白（CREB），这与增强的肿瘤细胞生长有关。