重组人IGF1R/CD221蛋白-ACROBiosystems百普赛斯

Human IGF1R / CD221 Protein, His Tag (MALS verified)

分子别名（Synonym）

IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216

表达区间及表达系统（Source）

Human IGF-I R, His Tag (IGR-H5229) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).

Predicted N-terminus: Glu 31

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.0 kDa and 23.8 kDa respectively. The protein migrates as 35-45 kDa (partial β chain), 110-120 kDa (α chain) and 130 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

胰岛素样生长因子1受体（IGF1R）也称为CD221、JTK13。并且是被IGF-1和相关生长因子IGF-2激活的跨膜受体。它属于一大类酪氨酸激酶受体。该受体介导IGF-1的作用，IGF-1是一种分子结构与胰岛素相似的多肽蛋白激素。IGF1R由两个α亚基和两个β亚基组成，α和β亚基都是由单个信使核糖核酸前体合成的。然后将前体糖基化，蛋白水解裂解，并通过半胱氨酸键交联，形成功能性跨膜αβ链。α链位于细胞外，而β亚基横跨细胞膜，负责配体刺激下的细胞内信号转导。IGF1R具有ATP的结合位点，ATP用于提供用于自身磷酸化的磷酸盐。IGF1R和胰岛素受体之间有60%的同源性。作为对配体结合的反应，α链诱导β链的酪氨酸自磷酸化。这一事件触发了细胞内信号的级联，虽然有一定的细胞类型特异性，但通常促进细胞存活和细胞增殖。