重组人整合素Integrin a4b7蛋白-ACROBiosystems百普赛斯

Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein, His Tag&Tag Free (MALS verified)

分子别名（Synonym）

Integrin alpha 4 beta 7,ITGA4&ITGB7

表达区间及表达系统（Source）

Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).

Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)

蛋白结构（Molecular Characterization）

Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB7). The protein migrates as 55 kDa, 80 kDa, 100-120 kDa and 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

整合素α4β7是一种整合素家族粘附受体，与α4β1（VLA4）和E-钙粘蛋白受体αEβ7共享亚基。整合素α4β7，对归巢肠粘膜至关重要，在Peyer斑块或肠系膜淋巴结的T细胞活化过程中被诱导。它的表达需要来自局部树突和基质细胞的信号，包括分泌的视黄酸。HIV-1包膜蛋白gp120与整合素α4β7的活性形式结合，这可以解释也可以不解释HIV-1病毒在肠道相关淋巴组织（GALT）中的浓度。整合素α4β7也可能参与急性肠移植物抗宿主病（GVHD）中的淋巴细胞运输。