Human FAP Protein, His Tag, active dimer (MALS verified)
分子别名（Synonym）
FAP,FAPalpha,SIMP,Seprase,APCE
表达区间及表达系统（Source）
Human FAP, His Tag (FAP-H5244) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).
Predicted N-terminus: His
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 86.9 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
FAP（也称为seprase）是一种II型跨膜丝氨酸蛋白酶。质膜和可溶性形式都表现出脯氨酸切割后的内肽酶活性，对Ala/Ser-Gly-Pro-Ser/Asn/Ala共有序列有显著的偏好。还可降解明胶，使I型胶原热变性。还具有二肽基肽酶活性，优选Ala-Pro、Ile-Pro、Gly-Pro、Arg-Pro和Pro-Pro。质膜形式与DPP4、PLAUR或整合素结合，参与细胞外基质（ECM）的细胞周蛋白水解，从而促进细胞通过ECM的粘附、迁移和侵袭。通过降解蛋白聚糖短肽促进神经胶质瘤细胞通过脑实质的侵袭。通过以丝氨酸蛋白酶活性独立的方式调节细胞增殖和存活，在黑素细胞中充当肿瘤抑制因子。
