重组人IL-13蛋白-ACROBiosystems百普赛斯

Human IL-13 Protein, His Tag, premium grade

分子别名（Synonym）

IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,Interleukin-13

表达区间及表达系统（Source）

Human IL-13 Protein, His Tag, premium grade (IL3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).

Predicted N-terminus: Gly 21

Human IL-13 Protein, His Tag, premium grade (IL3-H52H4), designed for preclinical stage, has the same activity and performance with GMP Human IL-13 Protein, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 14.2 kDa. The protein migrates as 15 kDa and 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素13（IL13）也称为ALRH、BHR1和P600，是一种单链糖基化多肽，是调节炎症和免疫反应的关键细胞因子。IL13由许多细胞类型分泌，但特别是由T辅助型2（Th2）细胞分泌。IL-13通过多亚基受体诱导其作用，该受体包括IL-4受体的α链（IL-4Rα）和两个已知的IL-13特异性结合链中的至少一个。IL-13的功能与IL-4的功能相当重叠，特别是在造血细胞诱导的变化方面，但考虑到IL-4更有效的作用，这些作用可能不那么重要。IL-13诱导基质金属蛋白酶（MMPs），作为防止易窒息的过度过敏性炎症机制的一部分。IL-13诱导过敏性肺病的许多特征，包括气道高反应性、杯状细胞化生和粘液分泌过多，这些都会导致气道阻塞。