重组链霉亲和素（Streptavidin）-ACROBiosystems百普赛斯

Recombinant Streptavidin Protein (MALS verified)

分子别名（Synonym）

Streptavidin,SA

表达区间及表达系统（Source）

Recombinant Streptavidin (STN-N5116) is expressed from E.coli cells.

蛋白结构（Molecular Characterization）

This protein carries no "tag". The protein has a calculated MW of 13.8 kDa.

应用说明（Application）

ELISA assay

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in NaCl, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 2 years in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

链霉亲和素是从阿维迪尼链霉菌中纯化的四聚体蛋白，对生物素具有很高的结合亲和力。能够将一个生物素分子与每个亚基结合。链霉亲和素（PI=6.0-7.5）在生理pH下与各种生物成分的非特异性结合水平低于抗生物素（PI=7.4），这是由其等电点（PI）引起的。链霉亲和素可用于亲和层析、ELISA、免疫组织化学和蛋白质印迹。