重组人APRIL/TNFSF13蛋白-ACROBiosystems百普赛斯

Human APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)

分子别名（Synonym）

APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2

表达区间及表达系统（Source）

Human APRIL Protein, His,Flag Tag (APL-H52D1) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # O75888-1) trimer Design.

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 50.2 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

APRIL（增殖诱导配体）也称为肿瘤坏死因子配体超家族成员13，TALL-2，TRDL-1，CD256，TNFFSF 13，与TNFRSF13B/TACI和TNFRSF17/BCMA结合的细胞因子。APRIL是主要与血液系统恶性肿瘤相关的肿瘤坏死因子家族的细胞因子。密切相关的TNF家族配体B细胞活化因子（BAFF）和增殖诱导配体（APRIL）用于成熟B淋巴细胞的产生和维持。BAFF和APRIL都组装成同源三聚体，结合并激活它们部分共享的几种受体。此外，APRIL的表达受miR-145在GC细胞中的调节。