重组人IL-4蛋白-ACROBiosystems百普赛斯

Human IL-4 Protein, premium grade

分子别名（Synonym）

IL4,BCGF1,BSF1

表达区间及表达系统（Source）

Human IL-4, premium grade (IL4-H4218) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1).

Predicted N-terminus: His 25

Human IL-4, premium grade (IL4-H4218), designed for preclinical stage, has the same activity and performance with GMP Human IL-4 (GMP-L04H26), which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 15.0 kDa. The protein migrates as 19 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

宿主蛋白残留（Host Cell Protein）

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素-4是一种诱导幼稚辅助性T细胞（Th0细胞向Th2细胞）分化的细胞因子。在存在IL-4和IL-13（Th-2型反应中产生的细胞因子，特别是在过敏和寄生虫感染期间）的情况下，巨噬细胞被差异激活，并且该细胞因子是白细胞介素4受体的配体。白细胞介素4受体也与IL13结合，这可能有助于该细胞因子和IL13的许多重叠功能。STAT6是一种信号转导和转录激活因子，已被证明在介导这种细胞因子的免疫调节信号中起着核心作用。最近，研究人员发现，细胞因子IL-4在几种基因缺陷小鼠品系（包括Itk，KLF2，CBP和Id3）的胸腺中先天CD8+T细胞的发育中起关键作用，而之前没有暴露于抗原。