重组人B7-H4蛋白-ACROBiosystems百普赛斯

Human B7-H4 Protein, His Tag (MALS verified)

分子别名（Synonym）

B7-H4,VTCN1,B7S1,B7h.5

表达区间及表达系统（Source）

Human B7-H4, His Tag (B74-H5222) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # NP_078902).

Predicted N-terminus: Phe 29

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.4 kDa. The protein migrates as 39-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

无菌（Sterility）

Negative

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

含V集结构域的T细胞活化抑制剂1（VTCN1）也称为免疫共刺激蛋白B7-H4、蛋白B7S1、T细胞共刺激分子B7x、B7H4，属于免疫球蛋白超家族和BTN/MOG家族。VTCN1包含两个Ig样V型（免疫球蛋白样）结构域。VTCN1的表达被IL6和IL10上调，并被抗原呈递细胞（APC）上的GM-CSF和IL4抑制。VTCN1/B7-H4通过抑制T细胞的活化、增殖、细胞因子的产生和细胞毒性的发展来负调控T细胞介导的免疫反应。VTCN1参与促进上皮细胞转化。