重组人Cathepsin B/CTSB蛋白-ACROBiosystems百普赛斯

Human Cathepsin B / CTSB Protein, His Tag (active enzyme, MALS verified)

分子别名（Synonym）

CTSB,CPSB,APPS

表达区间及表达系统（Source）

Human Cathepsin B, His Tag (CTB-H5222) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Ile 339 (Accession # P07858-1 (L26V)).

Predicted N-terminus: Arg 18 & Leu 80

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The Human Cathepsin B will be further processed into mature form (Leu 80-Ile 339). The protein has a calculated MW of 36.7 kDa (pro-form) and 29.5 kDa (mature-form). The protein migrates as 42-50 kDa and 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

组织蛋白酶B（CTSB）也称为APP分泌酶（APPS），CPSB是属于肽酶C1家族的酶促蛋白。组织蛋白酶B/CTSB被合成为前酶。去除信号肽后，无活性的酶原会进行进一步的修饰，包括去除前区以产生活性酶。组织蛋白酶B/APP的催化活性包括：对肽键具有广泛特异性的蛋白质水解；优先切割小分子底物中的-Arg-Arg-Xaa键（因此不同于组织蛋白酶L）；除了作为内肽酶外，还显示肽基二肽酶活性，释放C端二肽。组织蛋白酶B/CPSB作为一种巯基蛋白酶，被认为参与蛋白质的细胞内降解和更新，也与肿瘤的侵袭和转移有关。组织蛋白酶B的过度表达与食管腺癌和其他肿瘤有关。