重组人CD32B/FCGR2B蛋白-ACROBiosystems百普赛斯

Human Fc gamma RIIB/C (CD32b/c) Protein, His Tag (SPR & BLI & MALS verified)

分子别名（Synonym）

FCGR2B,C,CD32b,c,FcRII-b,c,Fc-gamma RII-b,c,Fc-gamma-RIIb,c,CD32,FCG2,IGFR2,CDw32

表达区间及表达系统（Source）

Human CD32b/c, His Tag (CDB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1). In the region Ala 46 - Pro 217, the AA sequence of Fc gamma RIIB and Fc gamma RIIC are homologus.

Predicted N-terminus: Ala 46

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.4 kDa. The protein migrates as 28-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）

IgG（FcγR）Fc区的受体是Ig超家族的成员，其在激活或抑制免疫应答中起作用。识别出三类人FcγR:RI（CD64），RII（CD32）和RIII（CD16），它们产生多种同种型。

人FcγRII/CD32有三个基因（A，B和C），小鼠FcγRII B有一个基因（CD32B）。低亲和力免疫球蛋白γFc区受体II-b（FCGR2B）也称为CD32b，FCG2，IGFR2。CD32B在B细胞和髓样树突状细胞上表达。CD32B在B细胞上的连接下调抗体产生，并且在某些情况下可能促进细胞凋亡。CD32B在树突状细胞上的共连接抑制成熟并阻断细胞活化。CD32B也可能是单克隆抗体治疗恶性肿瘤的靶标。