重组人FOLR1蛋白-ACROBiosystems百普赛斯

Human FOLR1 Protein, His Tag (MALS verified)

分子别名（Synonym）

FOLR-1,FBP,FOLR,FRα

表达区间及表达系统（Source）

Human FOLR1, His Tag (FO1-H52H1) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1).

Predicted N-terminus: Arg 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-40 KDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

叶酸受体1（FOLR1）也称为叶酸受体α、叶酸结合蛋白（FBP）、FOLR，是叶酸受体（FOLR）家族的成员。该基因家族的成员对叶酸和几种还原的叶酸衍生物具有高亲和力，并介导5-甲基四氢叶酸向细胞内部的递送。成熟FOLR1是一种N-糖基化蛋白，通过GPI连接锚定在细胞表面。FOLR1主要在上皮细胞上表达，在许多癌症上显著上调。FOLR1被内化到内涵体系统中，在回收到细胞表面之前，它与配体解离。可溶性形式的FOLR1可以从细胞表面蛋白水解脱落到血清和母乳中。FOLR1的缺陷是由于脑叶酸转运缺乏（NCFTD）引起的神经退行性变的原因。NCFTD是一种常染色体隐性遗传疾病，由早期大脑特异性叶酸缺乏引起。