重组人FCGR1/CD64蛋白-ACROBiosystems百普赛斯

Human Fc gamma RI / CD64 Protein, His Tag (SPR & BLI & MALS verified)

分子别名（Synonym）

FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI

表达区间及表达系统（Source）

Human CD64, His Tag (FCA-H52H1) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Leu 281 (Accession # P12314-1).

Predicted N-terminus: Gln 16

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 31.8 kDa. The protein migrates as 45-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）

识别IgG Fc部分的受体分为三组，分别称为Fcγ-RI、RII和RIII，也分别称为CD64、CD32和CD16。Fc-gamma RI以高亲和力结合IgG，并在早期免疫反应中发挥作用。Fc-gamma RII和RIII是低亲和力受体，在晚期免疫应答期间将IgG识别为多价抗原周围的聚集体。

高亲和力免疫球蛋白γ-Fc受体I也称为FCGR1A、FCG1、FCGR1、CD64和IGFR1，是一种以高亲和力结合单体IgG型抗体的完整膜糖蛋白，属于免疫球蛋白超家族或FCGR1家族。FCGR1A/CD64含有3个Ig样C2型（免疫球蛋白样）结构域。CD64仅在巨噬细胞和单核细胞上存在，但用IFNγ和G-CSF等细胞因子处理多形核白细胞可以诱导CD64在这些细胞上表达。