Human HGFR / c-MET Protein, His Tag
分子别名（Synonym）
MET,AUTS9,HGFR,RCCP2,c-Met
表达区间及表达系统（Source）
Human HGF R, His Tag (MET-H5227) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932 (Accession # AAI30421.1).
Predicted N-terminus: Glu 25
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus. The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa and 70.2 kDa. The protein migrates as 40-45 kDa and 85-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
肝细胞生长因子受体（HGFR）也被称为间质-上皮转化因子（MET），c-MET，是一种糖基化受体酪氨酸激酶，在上皮形态发生和癌症发展中起着核心作用。HGFR蛋白具有酪氨酸激酶活性。初级单链前体蛋白经翻译后切割产生α和β亚基，二硫键连接形成成熟受体。HGFR通常由上皮来源的细胞表达，而HGF的表达仅限于间充质来源的细胞。在HGF刺激下，HGFR诱导几种生物反应，共同产生一种称为侵入性生长的程序。癌症中HGFR的异常激活与预后不良相关，其中异常激活的HGFR触发肿瘤生长、为肿瘤提供营养的新血管形成（血管生成）以及癌症扩散到其他器官（转移）。HGFR在许多类型的人类恶性肿瘤中失调，包括肾癌、肝癌、胃癌、乳腺癌和脑癌。正常情况下，只有干细胞和祖细胞表达HGFR，然而，癌症干细胞被认为劫持了正常干细胞表达HGFL的能力，从而成为癌症持续存在并扩散到身体其他部位的原因。HGFR基因的各种突变与乳头状肾癌有关。HGFR介导一种称为侵袭性生长的复杂程序。HGFR的激活触发有丝分裂和形态发生。
