重组人Nectin-4蛋白-ACROBiosystems百普赛斯

Human Nectin-4 Protein, His Tag

分子别名（Synonym）

NECTIN4,LNIR, PRR4, PVRL4

表达区间及表达系统（Source）

Human Nectin-4, His Tag (NE4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).

Predicted N-terminus: Gly 32

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 36.0 kDa. The protein migrates as 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Nectin-4（基因名PVRL4，脊髓灰质炎病毒受体样4）是一种66kDa的I型跨膜糖蛋白，属于Ig超家族蛋白的Nectin家族。凝集素是细胞粘附分子，在上皮细胞、内皮细胞、免疫细胞和神经元细胞的发育和成年生活中，在各种生物过程中发挥关键作用，如极性、增殖、分化和迁移。在50%、49%和86%的乳腺癌、卵巢癌和肺癌中，凝集素-4是一种肿瘤相关抗原，主要用于预后不良的肿瘤。在相应的正常组织中未检测到其表达。