重组人PSMA/FOLH1蛋白-ACROBiosystems百普赛斯

Human PSMA / FOLH1 Protein, His Tag (active enzyme, MALS verified)

分子别名（Synonym）

FOLH1,PSMA,GIG27,FOLH,NAALAD1,PSM,NAALADase I,GCPII,FGCP

表达区间及表达系统（Source）

Human PSMA, His Tag (PSA-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # Q04609-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 81.4 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in MES and NaCl with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景（Background）

前列腺特异性膜抗原（PSMA）也称为叶酸水解酶1（FOLH1），谷氨酸羧肽酶2（GCP2），N-乙酰化-α-连接的酸性二肽酶I（NAALAD1），属于肽酶M28家族和M28B亚家族。FOLH1/PSMA在pH值大于6.5时稳定。FOLH1/PSMA是一种II型跨膜锌金属肽酶，在神经系统，前列腺，肾脏和小肠中表达最高。FOLH1/GCP-2是同型二聚体，每个亚基结合2个锌离子，是NAALase活性所必需的。PSMA的催化活性涉及释放未取代的C端谷氨酰残基，通常来自Ac-Asp-Glu或叶酸聚γ-谷氨酸。FOLH1/GCP-2/PSMA具有叶酸水解酶和N-乙酰化-α-连接的酸性二肽酶（NAALADase）活性，并且优先选择三α-谷氨酸肽。GCP-2/PSMA参与前列腺肿瘤进展，并且还表现出二肽基肽酶IV型活性。在体外，切割Gly-Pro-AMC。