重组人Glypican 3/GPC3蛋白-ACROBiosystems百普赛斯

Human Glypican 3 / GPC3 Protein, His Tag, premium grade

分子别名（Synonym）

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

表达区间及表达系统（Source）

Human Glypican 3, His Tag, premium grade (GP3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Predicted N-terminus: Gln 25 & Ser 359

该产品是在我们严格的质量控制体系下生产的，该体系包括一系列全面的测试，包括无菌和内毒素测试。在临床前早期阶段，产品性能经过仔细验证和测试，可用于细胞培养或任何其他应用。当准备进入后期临床阶段时，我们还提供定制GMP蛋白质服务，以满足您的需求。我们将与您合作，根据您的要求定制和开发GMP级产品，该产品也符合细胞疗法细胞制造中使用的原材料和辅助材料的要求。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively. The protein migrates as 30 kDa, 40 kDa and 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Glypican-3（GPC3）也称为肠蛋白OCI-5，GTR2-2，MXR7，属于磷脂酰肌醇蛋白聚糖家族。磷脂酰肌醇蛋白聚糖3/GPC-3在肺，肝和肾中高度表达。磷脂酰肌醇蛋白聚糖-3抑制DPP4的二肽基肽酶活性。磷脂酰肌醇蛋白聚糖-3可能参与抑制/调节主要中胚层组织和器官的生长，也可能在调节IGF2与其受体的相互作用中发挥作用，从而调节其功能。