重组人CD19蛋白-ACROBiosystems百普赛斯

PE-Labeled Human CD19 (20-291) Protein, His Tag (Site-specific conjugation)

分子别名（Synonym）

CD19,B4,CVID3,MGC12802

表达区间及表达系统（Source）

PE-Labeled Human CD19 (20-291), His Tag (CD9-HP2H3) is produced via site-specific conjugation of PE to Human CD19 (20-291), His Tag under optimal conditions with a proprietary technology. Human CD19 (20-291), His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).

Predicted N-terminus: Pro 20

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 33.7 kDa.

偶联（Conjugate）

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

应用说明（Application）

Evaluation of anti-CD19 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

B淋巴细胞抗原CD19也称为CD19（分化簇19），是一种单程I型膜蛋白，含有两个Ig样C2型（免疫球蛋白样）结构域。CD19在滤泡树突细胞和B细胞上表达。事实上，它存在于B细胞上，从发育过程中最早可识别的B谱系细胞到B细胞母细胞，但在成熟为浆细胞时丢失。它主要作为与CD21和CD81结合的B细胞共受体。激活后，CD19的细胞质尾部被磷酸化，导致Src家族激酶结合并募集PI-3激酶。与T细胞一样，几个表面分子形成抗原受体，并在B淋巴细胞上形成复合物。（几乎）B细胞特异性CD19磷酸糖蛋白就是这些分子之一。其他是CD21和CD81。这些表面免疫球蛋白（sIg）相关分子促进信号转导。在活的B细胞上，模拟外源性抗原的抗免疫球蛋白抗体使CD19与sIg结合并内化。相反的过程尚未得到证实，这表明这种受体复合物的形成是由抗原诱导的。这种分子关联已被化学研究证实。CD19突变与以抗体产生减少为特征的严重免疫缺陷综合征有关。CD19已被证明与CD81、CD82、补体受体2和VAV2相互作用。