重组人TL1A蛋白-ACROBiosystems百普赛斯

Biotinylated Human TL1A / TNFSF15 Protein, His, Avitag

分子别名（Synonym）

TL1A, VEGI, TNFSF15

表达区间及表达系统（Source）

Biotinylated Human TL1A Protein, His,Avitag (TLA-H52Q1) is expressed from human 293 cells (HEK293). It contains AA Leu 72- Leu 251 (Accession # O95150-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 24.1 kDa. The protein migrates as 28-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

TNF样细胞因子1A（TL1A）及其受体、死亡受体3（DR3）和诱饵受体3（DcR3）分别是TNF和TNF受体蛋白超家族的成员。APC衍生的TL1A与淋巴细胞DR3的结合为活化的淋巴细胞提供了共刺激信号。DR3信号不仅影响效应淋巴细胞的增殖活性和细胞因子的产生，还严重影响调节性T细胞的发育和抑制功能。然而，DcR3限制了TL1A/DR3复合物的功能：减弱T细胞活化并下调促炎细胞因子的分泌。与DR3和DcR3一起，TL1A构成了一个细胞因子系统，积极干扰免疫反应的调节。