重组人PD-1蛋白-ACROBiosystems百普赛斯

Human PD-1 / PDCD1 Protein, His Tag (MALS Verified)

分子别名（Synonym）

PDCD1,PD1,CD279,SLEB2

表达区间及表达系统（Source）

Human PD-1, His Tag (PD1-H5221) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2).

Predicted N-terminus: Leu 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.8 kDa. The protein migrates as 33-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）

程序性细胞死亡蛋白1（PD-1）也称为CD279和PDCD1，是一种I型膜蛋白，是T细胞调节因子CD28/CTLA-4扩展家族的成员。PDCD1在活化的T细胞、B细胞、巨噬细胞、髓细胞和胸腺细胞亚群的表面上表达。PD-1有两个配体，PD-L1和PD-L2，它们是B7家族的成员。经IFN-γ治疗后，PD-L1在几乎所有小鼠肿瘤细胞系上表达，包括PA1骨髓瘤、P815肥大细胞瘤和B16黑色素瘤。PD-L2的表达更受限制，并且主要由DC和一些肿瘤系表达。PD1通过抑制PI3K/AKT通路的激活和转导，抑制T细胞增殖和相关细胞因子的产生，包括IL-1、IL-4、IL-10和IFN-γ。此外，PD1的共聚合通过使关键信号转导子去磷酸化来抑制BCR介导的信号。在体外，用PD-L1-Ig处理抗CD3刺激的T细胞导致T细胞增殖和IFN-γ分泌减少。针对PD-1的单克隆抗体正在被开发用于治疗癌症，以增强免疫系统。