Human VEGF165-coupled Magnetic Beads
背景（Background）
人VEGF165偶联磁珠是通过将生物素化的人VEGF165%蛋白偶联到链亲和素偶联磁珠上来制备的。预偶联珠可用于以高特异性从样品中捕获抗VEGF165抗体或VEGFR2/KDR蛋白。珠粒的均匀尺寸和大的表面积与体积比确保了在简单、快速、方便的工作流程中高效的免疫捕获，并具有高的数据可重复性。
表达区间及表达系统（Source）
Human VEGF165 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191(Accession # P15692-4).
应用说明（Application）
This product is intended for immunocapture, biopanning and flow cytometry. This is a non-sterile product.
The Isotype control (Cat. No. SMB-B01) is sold separately.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Antibody Purification:
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
