CD19稳定细胞系/细胞株-ACROBiosystems百普赛斯

HEK293/Human Anti-CD19 Stable Cell Line

描述（Description）

HEK293/Human Anti-CD19 Stable Cell Line

应用说明（Application）

Binding assay by FACS and cell based ELISA

生长特性（Growth Properties）

Adherent

筛选标记（Selection Marker）

Puromycin (5 μg/mL)

培养基（Culture Medium）

DMEM medium + 10% FBS

冻存液（Freeze Medium）

10% DMSO + 90% FBS

装量（Quantity）

1 vial contains at least 5x10^6 cells in 1 ml of 10% DMSO in FBS.

存储（Storage）

Frozen in liquid nitrogen.

支原体检测（Mycoplasma Testing）

Negative

无菌检测（Sterility Testing）

Negative

使用说明（Instructions for Use）

See data sheet for detailed culturing and assay protocol.

背景（Background）

Chimeric antigen receptors (CARs) comprise interact domains, of which target cancer cell expressed antigens and allow T cell to efficiently kill the cancer cell. FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. Our CAR-like cells apply the HEK293 cells for the expression of FMC63 which will be presented on the cell membrane. It mimics the real CAR-T cell reaction and it is used as positive control for Anti-CD19 scFv Antibody flow cytometry detection.

Permits & Restrictions

This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.