ClinMax™ Human GM-CSF ELISA Kit
背景（Background）
ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human GM-CSF that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
原理（Assay Principles）
This assay kit is used to measure the concentrations of human Granulozyten-Makrophagen-Kolonie-stimulierende Faktor (GM-CSF) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- GM-CSF Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-GM-CSF Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
应用说明（Application）
The kit is developed for quantitative detection of GM-CSF in human serum and cell culture supernates.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
背景（Background）：GM-CSF
GM-CSF, Granulocyte Macrophage Colony Stimulating Factor GM-CSF is a colony-stimulating factor that regulates growth and differentiation of hematopoietic cells. GM-CSF is a primarily T cell-derived 14-35 kDa variably glycosylated protein which affects growth and functions of granulocytes and cells of the macrophage lineage at all stages of maturation.
It is also required for the development of specialized alveolar macrophages responsible for pathogen surveillance and the metabolism of pulmonary surfactant. Its production is widespread throughout the body in certain infectious and inflammatory states to enhance a local innate immune response. On the other hand, its abnormal production can lead to chronic inflammatory states as observed in rheumatoid arthritis and asthma. Some of the actions of GM-CSF include enhancing macrophage, neutrophil, eosinophil, and natural killer cell effector functions; dendritic cell survival and differentiation; and the up-regulation of antigen presentation.
