NFAT报告细胞系/细胞株-ACROBiosystems百普赛斯

NFAT (Luc) HEK293 Reporter Cell

描述（Description）

The NFAT (Luc) HEK293 Reporter Cell was engineered with the NFAT response element driving luciferase expressing systems. The receptors expressing endogenously or transfected on this reporter cell were activated by corresponding ligands binding, transducing intracellular signals resulting in NFAT-RE mediated luminescence.

应用说明（Application）

1. The discovery of activators or inhibitors by the NFAT signaling bioactivity

2. Transfection host for some receptors concerning the NFAT signaling pathway

生长特性（Growth Properties）

Adherent

筛选标记（Selection Marker）

Hygromycin (50 μg/mL)

培养基（Culture Medium）

DMEM medium + 10% FBS

冻存液（Freeze Medium）

Serum-free cell cryopreservation medium

装量（Quantity）

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

存储（Storage）

Frozen in liquid nitrogen.

支原体检测（Mycoplasma Testing）

Negative

无菌检测（Sterility Testing）

Negative

使用说明（Instructions for Use）

See data sheet for detailed culturing and assay protocol.

背景（Background）

Nuclear factor of activated T cells (NFAT), which is the pharmacological target of immunosuppressants cyclosporine and tacrolimus, has been shown to play an important role in T cells (immune system) and many biological events. Accumulating studies have indicated that NFATs are involved in many aspects of cancer, including carcinogenesis, cancer cell proliferation, invasion, metastasis, angiogenesis, drug resistance and tumor microenvironment. As the key section of the adaptive immune response, the T cell receptor (TCR)-calcium-calcineurin signaling pathway could lead to T cell activation. The 'nuclear factor of activated T cells' proteins are transcription factors that promote expression of a panel of genes required for activation.

Permits & Restrictions

This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.