EGFR报告细胞系/细胞株-ACROBiosystems百普赛斯

EGFR (Luc) HEK293 Reporter Cell

描述（Description）

The Human EGF R (Luc) HEK293 Reporter Cell was engineered to not only express STAT3 signaling response element, but also express the receptor full length human EGF R (Gene ID: 1956). When stimulated with human EGF protein, the EGF/EGF R interaction drives STAT3-mediated luminescence. Inhibition of EGF binding to EGF R by either anti-EGF or anti-EGF R antibodies results in a decrease in luminescence.

应用说明（Application）

1. Screen for anti-human EGF R or anti-human EGF neutralizing antibody.

2. Screen for human EGF R small molecule inhibitor

生长特性（Growth Properties）

Adherent

筛选标记（Selection Marker）

Hygromycin (40 μg/mL) + Puromycin (2 μg/mL)

培养基（Culture Medium）

DMEM medium + 10% FBS

冻存液（Freeze Medium）

Serum-free cell cryopreservation medium

装量（Quantity）

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

存储（Storage）

Frozen in liquid nitrogen.

支原体检测（Mycoplasma Testing）

Negative

无菌检测（Sterility Testing）

Negative

使用说明（Instructions for Use）

See data sheet for detailed culturing and assay protocol.

背景（Background）

The epidermal growth factor receptor (EGFR) belongs to the ErbB family of receptor tyrosine kinases (RTK). The aberrant activity of EGFR has shown to play a key role in the development and growth of tumor cells, where it is involved in numerous cellular responses including proliferation and apoptosis.

Permits & Restrictions

This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.