TSLP R报告细胞系/细胞株-ACROBiosystems百普赛斯

Human TSLP R (Luc) HEK293 Reporter Cell

描述（Description）

The Human TSLP R (Luc) HEK293 Reporter Cell was engineered to not only express STAT5 signaling response element, but also express the receptors full length human TSLP R (Gene ID: 64109) and IL-7 R alpha (Gene ID: 3575). When stimulated with human TSLP protein, the TSLP/TSLP R interaction drives STAT5-mediated luminescence. Inhibition of TSLP binding to TSLP R by either anti-TSLP or anti-TSLP R antibodies results in a decrease in luminescence.

应用说明（Application）

Screen for anti-human TSLP or anti-human TSLP R neutralizing antibody

生长特性（Growth Properties）

Adherent

筛选标记（Selection Marker）

Zeocin (100 μg/mL) + Puromycin (2 μg/mL)

培养基（Culture Medium）

DMEM medium + 10% FBS

冻存液（Freeze Medium）

Serum-free cell cryopreservation medium

装量（Quantity）

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

存储（Storage）

Frozen in liquid nitrogen.

支原体检测（Mycoplasma Testing）

Negative

无菌检测（Sterility Testing）

Negative

使用说明（Instructions for Use）

See data sheet for detailed culturing and assay protocol.

背景（Background）

Thymic stromal lymphopoietin (TSLP) is a pleiotropic cytokine and takes multi-functions via binding to a high-affinity heteromeric complex composed of thymic stromal lymphopoietin receptor (TSLPR) chain and IL-7Rα. The receptor complex on cells co-expressing TSLPR and IL-7Rα activated downstream genes expression and initiate proinflammatory signaling. TSLP involved in a variety of allergic diseases (e.g., atopic dermatitis, bronchial asthma). Further investigation indicated that TSLP is also involved in chronic inflammatory (i.e., chronic obstructive pulmonary disease and celiac disease) and autoimmune (e.g., psoriasis, rheumatoid arthritis) disorders and several cancers.

Permits & Restrictions

This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.