LG 培养

Usages:

For detection of molds, yeasts and total bacteria , commonly used in aseptic filling empty PET bottle production line of sterility testing.

Principle: peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; glucose carbon source; potassium dihydrogen phosphate as a buffer; provide essential trace elements of magnesium sulfate; ammonium sulfate osmotic equilibrium can be maintained and conducive to the growth target bacteria

Formulation (per liter):

Glucose :20.0g

Ammonium sulfate:2.0g

Magnesium sulfate:1.0g

Peptone:2.0g

Yeast extract powder: 3.5g

Sodium dihydrogen phosphate: 1.0g

Final pH 6.5 ± 0.2

How to use:

1. Weight  29.5g of product, adding 1L of  distilled water or deionized water,stirring heated to boiling until complete dissolution, dispensing tube or flask, 121 ℃ sterilization for 15minutes.

2. Inoculated the treated sample to LG medium, according to the specific requirements of sterility testing, vaccination appropriate sample size, the number of pieces of medium and positive control, the medium was placed in 28 ℃ ~ 32 ℃ prescribed time training 13 ~ 15d.

3. Observe the results.

Quality Control:

Appearance:

Powder: light yellow powder

Prepared broth: yellow, clear and transparent without precipitation.

Biology testing: The following strains were inoculated after 28 ℃ ~ 32 ℃ cultured 13 ~ 15d growth in the following table:

Quality control

Bacteria Name                 Bacteria No.                   growth characteristics

Pseudomonas aeruginosa        ATCC27853                       turbid broth

Staphylococcus aureus          ATCC6538                        turbid broth

Bacillus cereus                ATCC63303                       turbid broth

Klebsiella pneumonia           ATCC46117                       turbid broth

Hyphae of Aspergillus niger      ATCC16404             flocculent, liquid black spores can

Candida albicans               ATCC10231                       turbid broth

Specifications: 10kg / bag.

Storage: Store in a dark, cool and dry place,sealed the product immediately after use.

Storage period of three years