alpha Tubulin Mouse mAb (Ab169013) - Western Blot
All lanes: alpha Tubulin Antibody (Ab169013) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 50.0 kDa
Observed band size: 50.0 kDa
Exposure time: 60.0 s 

alpha Tubulin Mouse mAb (Ab169013) - Flow Cytometry
Overlay histogram showing HeLa cells stained with alpha Tubulin Mouse mAb (Ab169013) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

alpha Tubulin Mouse mAb (Ab169013) - ICC/IF
IF analysis of alpha Tubulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab169013) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.