aladdin 阿拉丁 P494193 pNPP Ready-to-use (RTU)

中文名：pNPP

英文名：pNPP

纯度：Ready-to-use (RTU)

货号：P494193

存储温度：2-8°C储存,避光

运输条件：低温运输

产品介绍：

Product Characteristics 

pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to pnitrophenol and inorganic phosphate. Liberated pnitrophenol is seen as a clear yellow color that is measured at 405/620 nm.

pNPP, liquid ready-to-use substrate, is highly active compared to pNPP ONE (cat. No. P494181). The substrate gives superior results due to its sensitivity, allowing very low detection limits.

Composition & Properties 

pNPP is a liquid, ready-to-use and aqueous formulation. The buffer solution contains stabilized pNPP and appropriate stabilizing components. Recommended stop solution is an equal volume of 0.1 M sodium hydroxide solution.

Working Procedure 

1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark. 

2.Recommended volumes are 100 µl substrate solution per microtiter well. Development time is typically 15- 30 minutes. Optimal time is determined by the user and will vary according to the procedure and the incubation temperature. 

3.100 µl of 0.1 M NaOH is then added and mixed thoroughly. 

4.In kinetic assays, the color is read directly at 405 nm. For endpoint assays the absorbance is read at 405/620 nm.

Tips & Tricks 

• For use in hot climate countries, due to superior background characteristics, we recommend pNPP ONE (cat. no. P494181) 

• A stop solution of 0.1 M NaOH requires no hazardous labelling. In case a higher assay sensitivity is required, a 1.0 M NaOH may be used as stop solution offering an approx. increase of OD of 3%. In both cases a 1 hour stop stability is guaranteed.

Handling & Storage

• Store solution at 2-8 °C in the dark. 

• Avoid exposure to light and heat. 

• Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color. 

Dispensing guidelines are available upon request.

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