中文名:快速内切酶ClaI
英文名:LightNing™ ClaI
货号:L359076
包装:1kit
存储温度:-20°C储存
产品介绍:
产品组成
货号 | 组分 | 规格 |
L359076A-50μl
| LightNing™ ClaI
| 50μl
|
L359076B-1ml
| 10×CutOne™ Buffer
| 1ml
|
L359076C-1ml
| 10×CutOne™ Color Buffer
| 1ml
|
产品简介
LightNing™ 快速内切酶是一系列经过基因工程重组、能够在 5~15 分钟内精确完成 DNA 切割的限制性内切酶,适用于质粒 DNA、 PCR 产物或基因组 DNA 等的快速酶切。LightNing™ 快速内切酶具有如下特点:5~15 分钟内即可完成酶切;共用一种酶切 Buffer,大大简化酶切反应体系;良好的酶活冗余度,轻松应对底物过量或困难模板酶切。此外,去磷酸化、连接试剂在 CutOne™ 酶切 Buffer 中具有 100% 活性,支持一管化反应,提升“酶切 - 修饰 - 连接” 的体验。
建议的反应条件
1× CutOne™ 缓冲液;
37℃温育;
参照“DNA 快速酶切流程”配制反应体系。
失活条件
80℃温育 20 min。
质量控制
1.功能活性检测:最适反应温度下,在 20 μl 反应体系中,1 μl LightNing™ ClaI 能够在 15 min 内完全消化 1 μg λDNA (Dam- )。
2.超长时间温育检测:最适反应温度下,将 1 μl LightNing™ ClaI 与 1 μg λDNA (Dam- ) 共同温育 3 h,未检测到其他核酸酶污染或星号活性引起的底物非特异性降解,延时酶切可能出现星号活性。
3.酶切 - 连接 - 再酶切检测:最适反应温度下,使用 1 μl LightNing™ ClaI 消化底物,回收酶切产物。在 22℃下使用适量 Fast T4 DNA Ligase 可以将酶切产物重新连接。将连接产物再次回收后,使用相同的内切酶可以重新切开连接产物。
4.非特异性内切酶活性检测:最适反应温度下,将 1 μl LightNing™ ClaI 与 1 μg 超螺旋质粒 DNA 共同温育 4 h,使用琼脂糖凝胶电泳检测,质粒 DNA 仍然处于超螺旋状态。
5.蓝白斑检测:将含有单一 lacZα 基因的载体以 1 μl LightNing™ BamHI 消化, 重新连接后转化入大肠杆菌感受态细胞,涂布在含有对应抗生素、 IPTG 和 X-gal 的 LB 培养基平板上。连接正确的产物会生长出蓝色菌落,而连接错误(即 DNA 末端切口不完整)的产物将得到白色菌落。 对于 LightNing™ 系列限制酶而言,白色菌落比例应小于 1%。
使用方法
1. DNA 快速酶切流程
① 在冰上按如下建议的加样顺序配制反应体系:
![](data:image/png;base64,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)
a. 本体系适用于经过纯化的 PCR 产物酶切。未纯化的 PCR 产物具备一定的离子强度,10× LightNing™ Buffer 加入量可适当减少至 2 μl。但由于 DNA 聚合 酶同时具有外切酶活性,会影响酶切产物,因此如下一步需进行克隆等操作,建议酶切前对 PCR 产物进行纯化。
② 轻柔吸打或轻弹管壁以混匀(切勿涡旋),然后瞬时离心以收集挂壁液滴;
③ 37℃温育 15 min(质粒),或 15~30 min(PCR 产物),或 30~60 min(基因组 DNA);
④ 80℃温育 20 min 即可使酶失活,停止反应(可选)。
2. 双酶切或多酶切
① 每种快速内切酶的用量为 1 μl,并根据需要适当扩大反应体系;
② 所有快速内切酶的体积总和不得超过总反应体系的 1/10
③ 如果所用的几种快速内切酶的最适反应温度不同,应先以最适温度低的酶开始酶切,再添加最适温度较高的酶,在其最适反应温度下进行酶切反应。
3. 适用于质粒的扩大反应体系
![](data:image/png;base64,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)
注:如果总反应体系大于 20 μl,应适当增加温育时间,尽量使用水浴、金属浴或沙浴。
不同 DNA 中的酶切位点数量
![](data:image/png;base64,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)
甲基化修饰影响
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在不同反应缓冲液中的活性![](https://aladdin-for-icloud-store.oss-cn-hangzhou.aliyuncs.com/private/product_template/359076/L359076_ms-5.png?access_token=efe412b1-3d98-4ef7-9529-bfcf8a894115)
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