aladdin 阿拉丁 F121201 FITC-CM-葡聚糖 150 kDa

中文名：FITC-CM-葡聚糖

英文名：FITC-CM-Dextran

中文别名：FITC-CM-葡聚糖 150, 异硫氰酸荧光素-羧甲基-右旋糖酐

纯度：150 kDa

货号：F121201

包装：200mg、500mg

存储温度：2-8°C储存,避光,充氩,干燥

产品介绍：

FITC-CM-dextrans are standard FITC-dextran carrying carboxymethyl-substituents. FITC-CM-dextrans have been developed for special studies on membranes and cells. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. Aladdin produce FITC-CM-dextrans from 4 kDa to 150 kDa. FITC-CM-dextran is supplied as a yellow powder.

Structure

FITC-CM-dextrans are manufactured by reacting selected dextran fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the dextran chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. The degree of substitution of FITC lies between 0.003 – 0.008.

Application

FITC-CM-dextran are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. The free carboxyl group is also useful for coupling other substances to the dextran chain.

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