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网站主页 RECOMBINANT CYTOKERATIN 20 ANTIBODY Recombinant Cytokeratin 20 Antibody
  • aladdin 阿拉丁 Ab098925 Recombinant Cytokeratin 20 Antibody Recombinant (RR648); Rabbit anti Human Cytokeratin 20 Antibody; WB, IHC, ICC, IF, Flow, IP; Unconjugated
  • aladdin 阿拉丁 Ab098925 Recombinant Cytokeratin 20 Antibody Recombinant (RR648); Rabbit anti Human Cytokeratin 20 Antibody; WB, IHC, ICC, IF, Flow, IP; Unconjugated
  • aladdin 阿拉丁 Ab098925 Recombinant Cytokeratin 20 Antibody Recombinant (RR648); Rabbit anti Human Cytokeratin 20 Antibody; WB, IHC, ICC, IF, Flow, IP; Unconjugated
  • aladdin 阿拉丁 Ab098925 Recombinant Cytokeratin 20 Antibody Recombinant (RR648); Rabbit anti Human Cytokeratin 20 Antibody; WB, IHC, ICC, IF, Flow, IP; Unconjugated
  • aladdin 阿拉丁 Ab098925 Recombinant Cytokeratin 20 Antibody Recombinant (RR648); Rabbit anti Human Cytokeratin 20 Antibody; WB, IHC, ICC, IF, Flow, IP; Unconjugated

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aladdin 阿拉丁 Ab098925 Recombinant Cytokeratin 20 Antibody Recombinant (RR648); Rabbit anti Human Cytokeratin 20 Antibody; WB, IHC, ICC, IF, Flow, IP; Unconjugated

Recombinant Cytokeratin 20 Antibody
￥499.90 10μl 起订
￥1299.90 50μl 起订
￥2099.90 100μl 起订
上海 更新日期：2025-05-16

上海阿拉丁生化科技股份有限公司

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联系人：阿拉丁客服
电话：400-6206333拨打
手机：13167063860 拨打
邮箱：anhua.mao@aladdin-e.com

产品详情:

中文名称：
Recombinant Cytokeratin 20 Antibody
英文名称：
Recombinant Cytokeratin 20 Antibody
品牌：
阿拉丁
产地：
上海
保存条件：
-20°C储存,避免反复冻融
纯度规格：
ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
产品类别：
抗体 流式抗体
分子式：
C60H103N2O13S4
分子量：
1286.75
运输条件：
超低温运输
产品规格：
100μl、50μl、10μl
货号：
Ab098925
是否进口：
否

基本信息

产品名称：Recombinant Cytokeratin 20 Antibody

规格或纯度：ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL

应用：WB, IF/ICC, IP, Flow Cytometry, IHC

特异性：Cytokeratin 20

种属反应性：人(Human)

预测反应种属：大鼠(Rat), 普通狨(Common marmoset), 山羊(Goat), 猪(Pig)

交叉反应：Human

免疫原：A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 20.

阳性对照：WB: HT-29 cell lysate. IHC: Colonic adenocarcinoma tissue. Flow: HT-29 cells. ICC/IF: HT-29 cells. IP: HT-29 whole cell lysate.

偶联：Unconjugated


产品属性

克隆类型：重组抗体

克隆号：RR648

Format：Whole IgG

亚型：Rabbit IgG

SDS-PAGE：150 kDa

纯化方法：Protein A purified

物理外观：Liquid

储存缓冲液：59% 10 mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.

防腐剂：0.01% Sodium azide

浓度：0.5 mg/mL

储存温度：-20°C储存,避免反复冻融

运输条件：超低温运输

稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。



功能和特点

宿主种属：兔(Rabbit)

种属反应性：人(Human)

克隆号：RR648

偶联：Unconjugated

图片

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Western Blot: Recombinant Cytokeratin 20 Antibody (Ab098925)

Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 0.1 s

>
IF/ICC: Recombinant Cytokeratin 20 Antibody (Ab098925)

Recombinant Cytokeratin 20 Antibody (Ab098925) - IF/ICC
Recombinant Cytokeratin 20 Antibody (Ab098925) staining Cytokeratin 20 in HT-29 cells by IF/ICC (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant Cytokeratin 20 Antibody (Ab098925) (1/200) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500).

>
Flow Cytometry : Recombinant Cytokeratin 20 Antibody (Ab098925)

Recombinant Cytokeratin 20 Antibody (Ab098925) - Flow Cytometry
Overlay histogram showing HT-29 cells stained with Recombinant Cytokeratin 20 Antibody (Ab098925) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant Cytokeratin 20 Antibody (Ab098925) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Blue) was used as a control.

>
IHC: Recombinant Cytokeratin 20 Antibody (Ab098925)

Recombinant Cytokeratin 20 Antibody (Ab098925) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of colonic adenocarcinoma tissue labelling Cytokeratin 20 with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

>
IP: Recombinant Cytokeratin 20 Antibody (Ab098925)

Recombinant Cytokeratin 20 Antibody (Ab098925) - IP
Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/50 dilution.
2nd Ab: G&R HRP for IP 1/500

Lane 1: Recombinant Cytokeratin 20 Antibody (Ab098925) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Cytokeratin 20 Antibody (Ab098925) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)

Exposure: 30.0 s.


查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab098925.html


anti- Cytokeratin 20抗体 ; 细胞角蛋白20抗体 ; 重组细胞角蛋白20抗体;CD20 antibody

公司简介

上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。 阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。

成立日期 (17年)
注册资本 14130.676万人民币
员工人数 500人以上
年营业额 ￥ 1亿以上
经营模式 工厂,试剂
主营行业 生物化工,化学试剂

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