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网站主页 RECOMBINANT CYTOKERATIN 18 ANTIBODY Recombinant Cytokeratin 18 Antibody
  • aladdin 阿拉丁 Ab098863 Recombinant Cytokeratin 18 Antibody Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab098863 Recombinant Cytokeratin 18 Antibody Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab098863 Recombinant Cytokeratin 18 Antibody Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab098863 Recombinant Cytokeratin 18 Antibody Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
  • aladdin 阿拉丁 Ab098863 Recombinant Cytokeratin 18 Antibody Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated

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aladdin 阿拉丁 Ab098863 Recombinant Cytokeratin 18 Antibody Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated

Recombinant Cytokeratin 18 Antibody
￥499.90 10μl 起订
￥1299.90 50μl 起订
￥2099.90 100μl 起订
上海 更新日期：2025-05-16

上海阿拉丁生化科技股份有限公司

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联系人：阿拉丁客服
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产品详情:

中文名称：
Recombinant Cytokeratin 18 Antibody
英文名称：
Recombinant Cytokeratin 18 Antibody
品牌：
阿拉丁
产地：
上海
保存条件：
-20°C储存,避免反复冻融
纯度规格：
ExactAb™, Validated, Recombinant, High performance, 0.5mg/mL
产品类别：
抗体 流式抗体
分子式：
C60H103N2O13S4
分子量：
1286.75
运输条件：
超低温运输
用途：
WB,Flow,IHC,IP,IF/ICC
产品规格：
100μl、50μl、10μl
货号：
Ab098863
是否进口：
否

基本信息

产品名称：Recombinant Cytokeratin 18 Antibody

规格或纯度：ExactAb™, Validated, Recombinant, High performance, 0.5mg/mL

应用：WB, IF/ICC, IP, Flow Cytometry, IHC

特异性：KRT18

应用：WB,Flow,IHC,IP,IF/ICC

种属反应性：人(Human), 大鼠(Rat)

交叉反应：Cross-reactivity with other species has not been measured.

免疫原：A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18 was used as an immunogen.

阳性对照：WB: Hela, A431, HepG2, T47D, MCF-7, A549 cell lysate; Rat Liver. IHC: Colonic adenocarcinoma tissue. Flow: HCT-116 cells. ICC/IF: HCT-116 cells. IP: Hela whole cell lysate.

偶联：Unconjugated


产品属性

抗体类型：Primary antibody

克隆类型：重组抗体

克隆号：RR693

Format：Whole IgG

亚型：Rabbit IgG

SDS-PAGE：150 kDa

纯化方法：Protein A purified

来源：CHO supernatant

物理外观：Liquid

储存缓冲液：59% 1*PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.

防腐剂：0.01% Sodium azide

浓度：0.5mg/mL

储存温度：-20°C储存,避免反复冻融

运输条件：超低温运输

稳定性与储存：在 -20°C 下可保存 2 年。收货后建议分装。避免冷冻/解冻循环。



功能和特点

宿主种属：兔(Rabbit)

种属反应性：人(Human), 大鼠(Rat)

克隆号：RR693

偶联：Unconjugated

图片

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Western Blot: Recombinant Cytokeratin 18 Antibody (Ab098863)

Recombinant Cytokeratin 18 Antibody (Ab098863) - Western Blot
All lanes: Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 44 kDa
Exposure time: 1.0 s

>
ICC/IF: Recombinant Cytokeratin 18 Antibody (Ab098863)

Recombinant Cytokeratin 18 Antibody (Ab098863) - ICC/IF
Recombinant Cytokeratin 18 Antibody (Ab098863) staining Cytokeratin 18 in HCT-116 cells by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1/2000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF488- conjugated Goat Anti-Rabbit IgG (1/500).

>
IHC: Recombinant Cytokeratin 18 Antibody (Ab098863)

Recombinant Cytokeratin 18 Antibody (Ab098863) - IHC
Immunohistochemistry analysis of colonic adenocarcinoma tissue labelling Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

>
Flow Cytometry: Recombinant Cytokeratin 18 Antibody (Ab098863)

Recombinant Cytokeratin 18 Antibody (Ab098863) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (Ab098863, 1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

>
IP: Recombinant Cytokeratin 18 Antibody (Ab098863)

Recombinant Cytokeratin 18 Antibody (Ab098863) - IP
Cytokeratin 18 was immunoprecipitated from 0.4mg of HeLa whole cell lysate with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/25 dilution.

Lane 1: Ab098863 IP in HeLa whole cell lysate
Lane 2: PBS instead of Ab098863 in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input)

Secondary: GAR HRP for IP 1:500
Exposure time: 20.0 s


查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab098863.html


Cytokeratin 18抗体 ; Cytokeratin 18兔抗体 ; CK18单克隆抗体 ; 重组CK18抗体

公司简介

上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。 阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。

成立日期 (17年)
注册资本 14130.676万人民币
员工人数 500人以上
年营业额 ￥ 1亿以上
经营模式 工厂,试剂
主营行业 生物化工,化学试剂

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