CDK5 Mouse mAb (Ab095769) - Western Blot
All lanes: CDK5 Mouse mAb (Ab095769) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 11.9 s

CDK5 Mouse mAb (Ab095769) - ICC/IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling CDK5 with CDK5 Mouse mAb (Ab095769) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

CDK5 Mouse mAb (Ab095769) - IHC
CDK5 Mouse mAb (Ab095769) staining CDK5 in human skeletal muscle sections by Immunohistochemistry (paraformaldehyde - fixed, paraffin - embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

CDK5 Mouse mAb (Ab095769) - Flow Cytometry
Overlay histogram showing K562 cells stained with CDK5 Mouse mAb (Ab095769) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by CDK5 Mouse mAb (Ab095769) (1/25 dilution) for 60 min at 37ºC. The secondary antibody was used Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 used under the same conditions. Acquisition of >10000 events was performed.