基本信息
产品名称：Caveolin-1 Antibody
规格或纯度：无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
应用：WB, IF/ICC, IHC
特异性：Caveolin-1
种属反应性：人(Human), 小鼠(Mouse), 猪(Pig)
免疫原：Recombinant CAV1 expressed in E.coli (AA 2-104)
阳性对照：WB: Mouse lung, A431 and A549 lysates and Mouse lung. IHC: Human Thyroid cancer tissue, Human Breast cancer tissue, Mouse Colon tissue, Mouse Cardiac Muscle tissue, Porcine Cerebrum tissue, Porcine Uterus tissue, Human Placenta tissue, Porcine Colon tissu
偶联：Unconjugated
产品属性
克隆类型：多克隆抗体
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Antigen affinity purified and protein A purified
物理外观：Liquid
储存缓冲液：10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂：0.05% Proclin 300
浓度：见COA
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 猪(Pig)
偶联：Unconjugated
图片
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Western Blot: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - Western Blot
All lanes: Caveolin-1 Antibody (Ab093242) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 19&21 kDa
Exposure time: 1.8 s
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Western Blot: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - Western Blot
All lanes: Caveolin-1 Antibody (Ab093242) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa
Exposure time: 1.8 s
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Samples: Human Thyroid cancer Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Mouse Colon Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Cerebrum Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Uterus Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Colon Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Uterus Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Small intestine Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Cardiac Muscle Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Lymph node Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Liver Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IHC
DAB staining on IHC
Sample: Porcine Lung Tissue
Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IF/ICC: Caveolin-1 Antibody (Ab093242)
Caveolin-1 Antibody (Ab093242) - IF/ICC
IF analysis of Caveolin-1 Antibody (Ab093242) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Caveolin-1 Antibody (Ab093242) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
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