APE1 Mouse mAb (Ab089159) - Western Blot
All lanes: APE1 Mouse mAb (Ab089159) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 35 kDa
Exposure time: 45.4 s

APE1 Mouse mAb (Ab089159) - IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling APE1 Mouse mAb (Ab089159) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).

APE1 Mouse mAb (Ab089159) - IHC
Immunohistochemical analysis of paraffin-embedded Human stomach section using APE1 Mouse mAb (Ab089159) at 1/50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

APE1 Mouse mAb (Ab089159) - IHC
Immunohistochemical analysis of paraffin-embedded human stomach sections labeling APE1 with APE1 Mouse mAb (Ab089159). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.