Sandwich ELISA - SHBG protein standard curve
SHBG protein was serially diluted 4-fold and captured by SHBG Mouse mAb (Ab005505) coated on a Clear Polystyrene Microplate (W291037). SHBG Mouse mAb (Ab005514) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).

Relevance of detection results between aladdin Antibody Pair (Ab005505 & Ab005514) and Beckman Coulter in CLIA
·Capture antibody: SHBG Mouse mAb (Ab005505)
·Detection antibody: SHBG Mouse mAb (Ab005514)

Binding ELISA - SHBG Mouse mAb (Ab005505)
Immobilized Recombinant Human SHBG Protein at 1.0 μg/mL can bind SHBG Mouse mAb (Ab005505) with the EC₅₀ of 11.47 ng/mL.