EndoS工具酶-ACROBiosystems百普赛斯

EndoS产品信息

名称：EndoS工具酶

物种：Streptococcus pyogenes serotype

属性：Protein

标记：Unconjugated

产品描述（Product Details）

This product is an IgG-specific endoglycosidase hydrolyzing complex N-glycans at the Fc N-glycosylation sites. It is derived from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 110 kDa.The enzyme deglycosylates IgG after the core GlcNAc and display limited activity on high-mannose and hybrid-type glycans.

应用说明（Application）

Endo S is specific for N-glycans attached on the Fc-domain of IgGs, and hydrolyzes Fc-glycans of all human IgG subclasses and IgG from many other species, including mouse, rat, monkey, sheep, goat, cow, and horse.

单位定义（Unit Definition）

One unit deglycosylates ≥ 95% of 1 μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH7.4 at 37°C for 30 min.

质量控制（Quility Control）

Less than 1.0 EU per μg by the LAL method.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

This product is stable after storage at:

The product MUST be stored at -20°C or lower upon receipt.

-20°C for 6 months under sterile conditions.

EndoS分子背景

EndoS is an endoglycosidase from Streptococcus pyogenes that specifically hydrolyzes N-linked glycans in the Fc-region of native IgG. It specifically deglycosylates the conserved N-glycans on the Fc region of IgG by hydrolyzing the chitobiose core (at the beta -1,4 linkage between the two N-acetylglucosamines) of the N-glycans, cleavage leave one GlcNAc residue remaining attached to the asparagine residue on the peptide backbone.