产品名称：Recombinant Human HRV 3C Protein

规格或纯度：无载体,生物活性,ActiBioPure™,无叠氮钠,His标签,≥95%(SDS-PAGE),见COA

应用：SDS-PAGE, Functional Studies

产品介绍：

HRV 3C蛋白酶是人鼻病毒3C (HRV 3C)的重组半胱氨酸蛋白酶，在大肠杆菌中表达并纯化。HRV 3C蛋白酶在Gln和Gly残基之间以识别序列Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro切割蛋白质底物。该蛋白酶的高特异性和高亲和力标签(6xHis)使其成为去除重组蛋白纯化和检测标签的理想选择，并能灵活去除该蛋白酶。
来源：
HRV 3C蛋白酶是在大肠杆菌中表达并纯化的一种人鼻病毒3C (HRV 3C)的重组半胱氨酸蛋白酶。
HRV 3C酶切His-GST-IL33蛋白，按照质量比（HRV 3C：目的蛋白）1：25和1：50酶切，4℃过夜酶切结果如下：完全酶切干净。

生物活性：2.5 Units/μg Unit definition:One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 95% completion at 4°C in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, pH 7.0.

纯度：>95% (SDS-PAGE)

氨基酸：1538-1719 aa

无载体：Yes

无动物源：No

Accession #：P03303

预测分子量：22.3 kDa

SDS-PAGE：24.0 kDa, reducing conditions; 24.0 kDa, non-reducing conditions

级别：无载体, 生物活性, ActiBioPure™, 无叠氮钠, His标签

储存缓冲液：10mM PBS, pH7.4

浓度：见COA

复溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存温度：-20°C储存,避免反复冻融

运输条件：超低温运输

稳定性与储存：-20℃保存12个月; 收货后建议分装 , 避免反复冻融。

Accession #：P03303

生物活性：2.5 Units/μg Unit definition:One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 95% completion at 4°C in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, pH 7.0.

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