Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 125.8 kDa
Exposure time: 6.879 s 

Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 122.0 kDa
Exposure time: 40.1 s 

Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 122.5 kDa
Exposure time: 0.1 s 

Vinculin Mouse mAb (Ab169192) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab169192) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Vinculin Mouse mAb (Ab169192) - ICC/IF
IF analysis of Vinculin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vinculin Mouse mAb (Ab169192) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.