GMP重组人Laminin 521蛋白-ACROBiosystems百普赛斯

GMP Human Laminin 521 Protein

产品描述（Product Details）

GMP Human Laminin 521 Protein (GMP Laminin 521) is a recombinant human protein that provides a defined surface for in vitro feeder-free culture of multiple human pluripotent stem cells (PSCs). GMP Laminin 521 has been proven to maintain normal growth characteristics and stemness in multiple PSC lines without simultaneous differentiation, which includes ESC, iPSC, MSC, etc. In addition, GMP Laminin 521 has been demonstrated to support PSC growth for >10 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages.

High-quality products and regulatory support files are essential for the smooth transition from preclinical research & development to cell therapy clinical study. Designed for clinical research, GMP Laminin 521 is manufactured by an animal-free process in a GMP-compliant facility. A full battery of QC testing is implemented to ensure product quality, including purity, bioactivity, sterility, mycoplasma, endotoxin, etc.

Flexible & Compatible

GMP Laminin 521 could work well in any commercial stem cell media. Meanwhile, it could support the attachment and expansion of hPSCs both in single cells or small colonies.

Stemness maintenance

GMP Laminin 521 is the biologically relevant hPSCs extracellular matrix. It is crucial for the growth and stemness maintenance of hPSCs in human through its binding to cell receptors a6β1 integrin.

Enhance cell differentiation

Due to the diverse biorelevant environment, GMP Laminin 521 could also enhance cell differentiation, polarization and organization of target cell types, including neurons, hepatocytes, cardiomyocytes, retinal pigmented epithelial cells, pancreatic β-cells, and so on.

Reduce Variability

GMP Laminin 521 is a defined, recombinant human protein with better lot-to-lot consistency that reduces variability in your PSC cultures.

优势特色（Features）

① Designed under ISO 9001:2015 and ISO 13485:2016

② Manufactured and QC tested under a GMP compliance factory

③ Animal-Free materials

④ Batch-to-batch consistency

⑤ Stringent quality control tests

产品参数（Key parameter）

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with blue ice, please inquire the shipping cost.

存储（Storage）

Upon receipt, store it immediately at -20°C or lower for long term storage.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 5 years in lyophilized state;

2. -70°C for 12 months under sterile conditions after reconstitution.

活性（Bioactivity）-Stem Cell Culture

Laminin 521分子背景

Laminin 521 were expressed in Descemet's membrane and corneal endothelium. This laminin isoform significantly enhanced the in vitro adhesion and proliferation, and differentiation of HCECs. Laminin 511-E8 consists of the C-terminal end of the alpha 5, beta 2, and gamma 1 chains.