LIF重组蛋白-ACROBiosystems百普赛斯

LIF产品信息

英文名称：Leukemia inhibitory factor

中文名称：白血病抑制因子

靶点别称：LIF,CDF,DIA,HILDA,MLPLI

物种：Human/Mouse/Rat

属性：Protein

标记：Biotin-labeled/Unconjugated

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

无菌（Sterility）

The sterility testing was performed by membrane filtration method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

LIF用户评价

LIF分子背景

白血病抑制因子（LIF）是一种白细胞介素6类细胞因子，是细胞中影响细胞生长和发育的蛋白质。白血病抑制因子具有多种功能，如胆碱能神经元分化、控制干细胞多能性、骨和脂肪代谢、因子依赖性细胞系的有丝分裂和促进体内巨核细胞的产生。

LIF的去除推动干细胞分化，但它们保留了增殖潜力或多能性。因此，LIF用于小鼠胚胎干细胞培养。有必要将干细胞保持在未分化状态，但是胚胎干细胞的基因操纵允许LIF非依赖性生长，特别是Nanog基因的过表达。人胚胎干细胞培养不需要LIF。