PH20重组蛋白-ACROBiosystems百普赛斯

PH20产品信息

英文名称：T-cell surface antigen CD2

靶点别称：SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15

物种：Human

标签：Tag Free / His Tag

属性：Protein

标记：Unconjugated

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

PH20分子背景

透明质酸酶PH20也称为精子粘附分子1（SPAM1）和精子表面蛋白PH-20，属于糖基水解酶56家族，SPAM1/PH-20在睾丸中表达。透明质酸中N-乙酰基-β-D-葡糖胺和D-葡萄糖醛酸残基之间（1->4）键的SPAM-1/PH20随机水解。SPAM-1/PH20参与精卵粘附。受精后，精子必须首先穿透卵子周围的一层卵丘细胞，然后才能到达透明带。卵丘细胞包埋在排卵前形成的含有透明质酸的基质中。SPAM1通过消化透明质酸帮助穿透卵丘细胞层。