KRAS重组蛋白，ACROBiosystems百普赛斯

KRAS产品信息

英文名称：V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

中文名称：V-Ki-ras2 Kirsten大鼠肉瘤病毒癌基因同源蛋白

靶点别称：KRAS，V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

物种：Human

属性：Protein

标记：Unconjugated

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl, 1 mM TCEP, pH 7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

KRAS分子背景

KRAS（Kirsten大鼠肉瘤2病毒癌基因同源物）基因是一种原癌基因，编码一种名为KRAS的小GTP酶转导蛋白。KRAS也被称为Ki-Ras、c-K-Ras和c-Ki-Ras。Ras蛋白结合GDP/GTP并具有内在的GTP酶活性。在调节细胞增殖中发挥重要作用，通过以ZNF304依赖的方式诱导癌症（CRC）细胞中肿瘤抑制基因（TSG）的转录沉默来促进致癌事件。RAS是人类癌症中最常见的突变癌基因之一，但RAS基因突变的频率和分布并不均匀。具体来说，甘氨酸12（G13）的突变通过干扰GAP结合和GAP刺激的GTP水解引起RAS激活。参考文献表明，该途径可能成为潜在的治疗靶点。